PCR conditions (AT):
AT = Annealing Temperature
Provides a link to the article where the PCR condition are mentioned
· Polymorphism Information Index (PIC) or Nei's diversity index is calculated as 1 – Σ (allele frequency)2 based upon the unique genotypes.
· Allelic diversity (h) = 1 – [n/(n-1)] Σi xi 2 where xi is the frequency of the ith allele at the locus and n the number of isolates (Appl Environ Microbiol 1986 May;51(5):873-84)
1= low resolution electrophoresis: agarose gel
2= acrylamide gel
3= high resolution (capillary electrophoresis…)